MFGE8 Antibody

SKU: orb1252491

Description

MFGE8 Antibody

Research Area

Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blot: 1-2 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Lactadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: EDM45
• Immunogen: A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.
• Target: MFGE8
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Lactadherin short form, Medin, MFGE8

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Lactadherin antibody (EDM45).

FFPE human lung carcinoma tested with Lactadherin antibody (EDM45).

FFPE human renal cell carcinoma tested with Lactadherin antibody (EDM45).

FFPE human pancreas tested with Lactadherin antibody (EDM45).

Western blot testing of MCF7 cell lysate with Lactadherin antibody (clone EDM45).

Quick Database Links

Gene Symbol

MFGE8

UniProt

Q08431