Pol Antibody

SKU: orb345071

Description

L1/ORF2 antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:20,000-1:100,000
• Reactivity: Mouse
• Application Notes:
  L1/ORF2 Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149.5 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with three synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
• Target: Pol
• Purity: Anti-L1/ORF2 Antibody is directed against three regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against mouse L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis shows reactivity with L1/ORF2 proteins from mouse sources based on 100% homology with the immunizing sequences. Cross reactivity against L1/ORF2 from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.21 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2

Quick Database Links

Gene Symbol

Pol

UniProt

P11369

RefSeq:AAC72810.1