L189

SKU: orb1221922

Description

L189 is a DNA ligase I, III and IV inhibitor (IC50 values are 5, 9 and 5 μM respectively) that blocks DNA binding. L189 inhibits base excision repair (BER) and non-homologous end joining (NHEJ). L189 specifically sensitizes cancer cells to DNA damage and increases the cytotoxicity of DNA-damaging agents. L189 will not only provide insights into the cellular function of these enzymes but also serve as lead compounds for the development of anticancer agents.

Key Properties

• CAS Number: 64232-83-3
• MW: 246.29
• Purity: >98% (HPLC)
• Formula: C₁₁H₁₀N₄OS
• SMILES: Nc1[nH]c(=S)[nH]c(=O)c1\N=C\c1ccccc1
• Target: DNA Ligase I| DNA Ligase III| DNA Ligase IV
• Solubility: DMSO : ≥ 33 mg/mL; 33.99 mM

Bioactivity

• In Vitro:
  L189 inhibits DNA Ligase I, III and IV activity with IC50 values of 5 μM, 9 μM and 5 μM, respectively. L189 (5 μM, 48 h) bring good anti-proliferation activity preferentially and cause cell death by creating a cytotoxic environment. L189 (5 μM, 48 h) reduces HeLa nuclear staining along with TMZ. L189 (5 μM, 48 h) enhances TMZ-induced HeLa growth arrestwhen along with TMZ that possibly in G2/M cell cycle phase without employing cell death mechanisms. Cell Cytotoxicity Assay Cell line: HeLa cells. Concentration: 5 μM. Incubation time: 48 h. Result: Blocked to HeLa growth and proliferation along with TMZ and not mark the significant cell cytotoxicity alone.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L189 | L 189 | L-189

Quick Database Links

Gene Symbol

DNA Ligase I| DNA Ligase III| DNA Ligase IV