L-Selenomethionine

SKU: orb1221017

Description

L-Selenomethionine is a naturally occurring amino acid. It promotes cell cycle progression and elevates the expression of the antioxidant enzymes thioredoxin reductase glutathione reductase.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 3211-76-5
• MW: 196.1
• Purity: >98% (HPLC)
• Formula: C₅H₁₁NO₂Se
• SMILES: C[Se]CC[C@H](N)C(O)=O
• Target: Amino Acids and Derivatives
• Solubility: DMSO: 10 mM;Water: 50 mg/mL (254.96 mM)

Bioactivity

• In Vivo:
  L-SelenoMethionine (0.06-12 μg/g diet; p.o. for 3 d) partially or completely prevent the decrease in the serum or plasma levels of total antioxidants in rats exposed to gamma rays, protons or HZE particles.
• In Vitro:
  L-SelenoMethionine (1-500 μM; 24-72 h) is selectively inhibits the growth of prostate cancer cells, compared with normal cells. L-Seleno Methionine (500 μM; 48 h) induce apoptosis in prostate cancer cells. L-SelenoMethionine (500 μM; 48 h) causes an increase in arrest in the G2-M phase of the cell cycle selectively in prostate cancer cells. L-SelenoMethionine (5 μM; 24 h) mitigates gene expression associated with the cellular stress response from 10 cGy irradiation. Cell Viability Assay Cell line: Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt). Concentration: 1, 5, 10, 50, 100, 500 μM. Incubation time: 24, 48, 72 hours. Result: Inhibited the growth of prostate cancer cells, with a lower IC50 (1-90 μM) at 72 h than normal prostate cells (>500 μM). Apoptosis Analysis Cell line: Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt. Concentration: 500 μ. Incubation time: 48 hours. Result: Exhibited the highest level of DNA condensation in androgen-responsive LNCaP carcinoma cells, followed by PC-3 and DU145 cells. Exhibited the nicked-end DNA labeling in prostate cancer cells. Promoted the PARP cleavage in prostate cancer cells. Cell Cycle Analysis Cell line: Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt). Concentration: 500 μM. Incubation time: 48 hours. Result: Increased the sub-G0-G1 cell fraction of LNCaP (41.5%), PC-3 (12.1%), and DU145 cells (11.2%). Caused a significant increases in G2 cells, particularly in LNCaP (13%), PC-3 (32%), and DU145 (20%) cells. RT-PC R Cell line: Human thyroid epithelial cells (HTori-3). Concentration: 5 μM. Incubation time: 24 hours. Result: Downregulated the CDC6, GADD45A, FAS and ATF3 in irradiated cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Amino Acids and Derivatives