KY1220

SKU: orb1308149

Description

KY1220

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 292168-79-7
• MW: 314.32
• Purity: 99.66%
• Formula: C₁₄H₁₀N₄O₃S
• SMILES: [O-][N+](=O)c1ccc(cc1)-n1cccc1\C=C1/NC(=S)NC1=O
• Target: Wnt/beta-catenin
• Solubility: DMSO:100 mg/mL (318.15 mM)

Bioactivity

• Target IC50:
  Wnt/β-catenin:2.1 μM
• In Vitro:
  KY1220 accelerate the degradation rates of both β-catenin and Ras in SW480 cell lines. KY1220 dose-dependently reduces Wnt3a-CM-induced TOPflash Reporter activation and mRNA expression of Wnt target genes CCND1 and MYC in HEK293 cells. Ras destabilization by KY1220 consequently inhibit the activities of both ERK and Akt, which are downstream effectors of Ras in SW480 cells harboring a KRAS mutation. In HEK293 cells, both β-catenin and pan-Ras protein levels are similarly reduced in a dose-dependent manner after treatment with KY1220, wherea the mRNA levels of CTNNB1 (which encodes β-catenin), NRAS, KRAS, and HRAS remain unchanged. K-Ras, which has a critical role in the progression of CRCs, is also destabilized by KY1220 via polyubiquitin-dependent proteasomal degradation the proliferation and transformation o the HCT15, SW480, D-WT, and D-MT CRC cells are efficiently inhibited after treatment with KY1220.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Wnt, Wnt/b-catenin, Wnt/betacatenin, Wnt/β-catenin, βcatenin, β-catenin, KY 1220, KY1220, KY-1220, inhibit, Inhibitor, HEK293 reporter cells, bcatenin, Beta catenin, beta-catenin, betacatenin

Compound Identifiers

PubChem CID

6011878