KY-02111

SKU: orb1306628

Description

KY02111 is a small molecule Wnt signaling inhibitor that promotes the differentiation of human pluripotent stem cells (hPSCs) into cardiomyocytes, acting downstream of GSK3β and APC. It is a valuable tool for in vitro cardiac differentiation studies and research into heart development and disease modeling.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1118807-13-8
• MW: 376.86
• Purity: >98%
• Formula: C₁₈H₁₇ClN₂O₃S
• SMILES: COc1ccc(CCC(=O)Nc2nc3ccc(Cl)cc3s2)cc1OC
• Target: Transcription factor
• Solubility: Soluble in DMSO (up to 40 mg/ml) or in Ethanol (up to 5 mg/ml with warming).

Bioactivity

• In Vivo:
  When applied to human embryonic stem cell lines (hESC; KhES-1 and KhES-3), human-induced pluripotent stem cell lines (hiPSC; 253G1, IMR90-1, IMR90-4, and RCHIPC0003), and a mouse embryonic stem cell line (R1), KY02111 (10 µM) significantly increases the proportion of cardiomyocytes by 70%-94%. In IMR90-1 hiPSCs, KY02111 (10 µM) downregulates the expression of 72.7% of target genes in the WNT signaling pathway. Furthermore, in TOPflash assays, a dose-dependent decrease in luciferase reporter activity was observed when KY02111 (10 µM) was applied to HEK293 and IMR90-1 hiPSC cells, indicating a suppression of WNT/β-catenin signaling. Remarkably, doses of KY02111 ranging from 10 µM to 25 µM enhanced cardiac differentiation in transgenic monkey ESCs approximately 80-fold compared to the control group, without exhibiting cytotoxicity at high concentrations. In SW480 cells, KY02111 (10 µM) also significantly reduced luciferase activity in TOPflash assays, highlighting its ineffectiveness against XAV939 and IWP-2. However, KY02111 notably diminished the luciferase activity induced by the GSK3β inhibitor BIO in SW480 cells, outperforming the effects of XAV939 and IWP-2.

Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cells, beta-catenin, betacatenin, differentiation, cardiac, cardiomyocyte, bcatenin, Inhibitor, inhibit, KY02111, KY-02111, KY 02111, pluripotent, of human, Wnt, Wnt/b-catenin, Wnt/β-catenin, Wnt/betacatenin, regeneration, stem, βcatenin

Compound Identifiers

PubChem CID

8582409