KRT8 Antibody / Cytokeratin 8

• Catalog Number: orb750219
• Category: Antibodies
• Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A cytoskeleton preparation containing keratin 8 was used as the immunogen for this Cytokeratin 8 antibody.
• UniProt ID: P05787
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM538
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cytokeratin 8 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of fixed human MCF7 cells with Cytokeratin 8 antibody (clone SPM538, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 8 antibody (clone SPM538).

Flow cytometry testing of MeOH fixed human HeLa cells with Cytokeratin 8 antibody (clone SPM538); Red=isotype control, Blue= Cytokeratin 8 antibody.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 8 antibody (clone SPM538) as confirmation of integrity and purity.