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Catalog Number | orb1254673 |
---|---|
Category | Antibodies |
Description | KRT7 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 51kDa |
Target | KRT7 |
UniProt ID | P08729 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Keratin, type II cytoskeletal 7, Cytokeratin-7, CK Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of HeLa cells, using KRT7 antibody (orb1254673). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (orb1254673) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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