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Catalog Number | orb606505 |
---|---|
Category | Antibodies |
Description | CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt] |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rOV-TL12/30 |
Tested applications | FACS, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody. |
Dilution range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Cytokeratin 7 antibody is available for research use only. |
UniProt ID | P08729 |
Storage | Store the recombinant Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The stated application concentrations are suggested starting amounts. Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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