KRT20/CK20/Cytokeratin 20 Antibody

SKU: orb1538356

Description

KRT20/CK20/Cytokeratin 20 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF (10 µg/ml), IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human
• Application Notes:
  Further information: Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 2G3-1C8
• Immunogen: KRT20 (AAH31559, 1 aa ~ 425 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: KRT20 / CK20 / Cytokeratin 20
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT20, Cytokeratin-20, Cytokeratin 20, CK20, K20, Keratin 20, Keratin-20, KRT21, CK-20, Protein IT

Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell (antibody concentration 10 ug/ml).

Western blot of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody, clone 2G3-1C8.

Detection limit for recombinant GST tagged KRT20 is approximately 0.03 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

KRT20 / CK20 / Cytokeratin 20