Keratin 19 Antibody

SKU: orb385888

Description

Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Cytokeratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [Wiki]

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the Keratin 19 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CTKN19-1
• Immunogen: Recombinant human KRT19 protein was used as the immunogen for the Keratin 19 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1, green) and Reddot nuclear stain (red).

Flow cytometry testing of methanol-fixed human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1); Red = isotype control, Blue = Cytokeratin 19 antibody.

Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone CTKN19-1). Predicted molecular weight ~43 kDa.

IHC testing of FFPE human pancreas and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human ovarian carcinoma and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Quick Database Links

UniProt

P08727