KRT18/CK18/Cytokeratin 18 Antibody

SKU: orb1535294

Description

KRT18/CK18/Cytokeratin 18 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.1 - 0.3 µg/ml)
• Reactivity: Bovine, Equine, Guinea pig, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kD according to NP_000215.1). Recommended concentration: 0.1-0.3 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human KRT18
• Target: KRT18 / CK18 / Cytokeratin 18
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT18, CK-18, Cytokeratin 18, Cytokeratin-18, K18, Keratin 18, CYK18, Keratin-18

KRT18 antibody (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

KRT18 / CK18 / Cytokeratin 18