KRT15 Antibody / Cytokeratin 15

• Catalog Number: orb639964
• Category: Antibodies
• Description: Cytokeratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2c, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the Cytokeratin 15 antibody.
• UniProt ID: P19012
• Tested applications: IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CTKN15-1
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone CTKN15-1, green) and Reddot nuclear stain (red).

IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone CTKN15-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone CTKN15-1). Expected molecular weight ~50 kDa.