KREMEN2 Antibody

• Catalog Number: orb1238862
• Category: Antibodies
• Description: KREMEN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-KREMEN2 antibody (orb1238862) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2. The immunogen is located within amino acids 390-440 of KREMEN2.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; ICC: 5 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 49kDObserved: 49kD
• Target: KREMEN2
• UniProt ID: Q8NCW0
• NCBI: NP_757384
• Storage: KREMEN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Di
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: KREMEN2, orb1238862 (2 µg/mL), KREMEN2, orb1271002 (1 µg/mL) and beta-actin, orb1240312 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: KREMEN2, orb1238862 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: KREMEN2 orb1238862 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of KREMEN2 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (orb1238862) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.