KRAS Antibody: HRP

• Catalog Number: orb535743
• Category: Antibodies
• Description: Rabbit polyclonal antibody against KRAS conjugated to HRP
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rat
• Immunogen: Synthetic peptide from the C-terminal of Human KRAS (aa. 100-189)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: HRP
• MW: 29 kDa
• Target: KRAS
• Entrez: 3845
• UniProt ID: P01116
• NCBI: NP_004976.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Transforming protein p21 antibody, c-Ki-ras antibo
• Note: For research use only
• Application notes: #REF!
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band (s): ~29 kDa (PTM).