KPNA6 Antibody

• Catalog Number: orb1246533
• Category: Antibodies
• Description: KPNA6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-STTGESVITREMVE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 60.0kDa according to NP_036448.1). Recommended concentration:0.1-0.3ug/ml. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.Immunohistochemistry:In paraffin embedded Human Adrenal Gland shows staining of cytoplasm and some nuclear membrane (nuclear pores). Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KPNA6
• UniProt ID: O60684
• NCBI: NP_036448.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: KPNA6 , karyopherin alpha 6 (importin alpha 7) , F
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 60.0kDa according to NP_036448.1). Recommended concentration:0.1-0.3ug/ml. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.Immunohistochemistry:In paraffin embedded Human Adrenal Gland shows staining of cytoplasm and some nuclear membrane (nuclear pores). Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246533 (0.1 ug/ml) staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246533 (3.8 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.