KPNA6 Antibody

SKU: orb1246533

Description

KPNA6 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 60.0kDa according to NP_036448.1). Recommended concentration:0.1-0.3µg/mL. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.Immunohistochemistry:In paraffin embedded Human Adrenal Gland shows staining of cytoplasm and some nuclear membrane (nuclear pores). Recommended concentration, 3-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-STTGESVITREMVE
• Target: KPNA6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KPNA6 , karyopherin alpha 6 (importin alpha 7) , FLJ11249, IPOA7, KPNA7, MGC17918, importin alpha 7 subunit, importin-alpha-S2, karyopherin alpha 6

orb1246533 (0.1 ug/ml) staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246533 (3.8 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KPNA6

UniProt

O60684

RefSeq (Protein):NP_036448.1