[KO] EGFR Rabbit mAb

• Catalog Number: orb1563546
• Category: Antibodies
• Description: [KO] EGFR Rabbit mAb
• Target: EGFR
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 0.3 mg/ml
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
• Purification: Affinity Purified
• Immunogen: A synthetic peptide of human EGFR
• UniProt ID: P00533
• MW: Calculated MW: 134 kDa; Observed MW: 175 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, IP, WB
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
• Antibody Type: Primary Antibody
• Clone Number: R01-2D3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR; ERBB; ERBB1; HER1; Epidermal growth factor r
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Western blot analysis of EGFR in Hela lysates using EGFR antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.