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What are Knockdown Validated Antibodies?
Knockdown-validated antibodies are used to ensure specific and accurate detection of target proteins in various biological and biomedical research applications. They are particularly valuable in experiments where antibody specificity is critical.
Antibody manufacturers such as Biorbyt are able to perform a negative control validation for antibodies such as knockdown validation. This technique involves the introduction of small interfering RNA (siRNA) to genetically modify target genes, “knocking down” their ability to produce the target protein.
The antibody can then be introduced to a treated tissue sample and should, if the validation is successful, return a lower (or null) reading for the presence of the target protein under Western blot, IHC and so on.
If the antibody performs in this expected manner, alongside other positive control validations, we can more safely assume that the antibody is specific to the target protein and therefore better able to ensure reliable, reproducible results under test conditions.
Benefits
- Promotes reproducibility of results
- Reduced background noise
- Greater confidence in results
- Minimizes risk of wasted samples
However, the technique does come with certain drawbacks, primarily that it is both time consuming and fairly difficult to perform, adding a further layer of complexity and delay to experiments. As such, many researchers choose instead to purchase knockdown validated antibodies directly from manufacturers such as Biorbyt. This allows them to commence experimentation, safe in the knowledge that the antibodies involved have already been validated, saving time, money and the potential for errors with the siRNA knockdown validation phase of their work.
Some of our most popular Knockdown Validated Antibodies
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