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Item 1 of 11
Item 1 of 11
Phospho-Akt (Thr450) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559195
| Catalog Number | orb559195 |
|---|---|
| Category | Antibodies |
| Description | Phospho-Akt (Thr450) Recombinant Rabbit Monoclonal Antibody |
| Target | AKT1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Akt around the phosphorylation site of Thr450 IT(p-T)PP |
| UniProt ID | P31749 |
| MW | 56 kDa |
| Tested applications | IF, IHC-Fr, IHC-P |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 4A3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | AKT1(phospho-Thr450); AKT1(phospho T450); AKT 1; A Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-Akt (Thr450) Monoclonal Antibody, Unconjugated (orb559195) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
