KLK2 Antibody

SKU: orb1247332

Description

KLK2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in Human Prostate lysates (calculated MW of 24.7kDa according to NP_001002231.1). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:In paraffin embedded Human Prostate shows vesicular staining in the secretory cells. Recommended concentration: 2.5-3.8µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KHQSLRPDEDSSHD
• Target: KLK2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KLK2, kallikrein-related peptidase 2, KLK2A2, MGC12201, hK2 , glandular kallikrein 2, kallikrein 2, prostatic

orb1247332 (0.1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247332 (3.8 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KLK2

UniProt

P20151

RefSeq (Protein):NP_001002231.1, NP_005542.1