KLF9 Antibody (N-term)

SKU: orb1935038

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse, Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD57562
• Immunogen: This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9. Antigen Region: 29-57 aa.
• Target: KLF9
• Molecular Weight: 27235 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Krueppel-like factor 9, Basic transcription element-binding protein 1, BTE-binding protein 1, GC-box-binding protein 1, Transcription factor BTEB1, KLF9, BTEB, BTEB1

KLF9 Antibody (N-term) western blot analysis in WiDr cell line lysates (35 ug/lane). This demonstrates the KLF9 antibody detected the KLF9 protein (arrow).

Anti-KLF9 Antibody (N-term) at 1:2000 dilution + U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-KLF9 Antibody (N-term) at 1:1000 dilution. Lane 1: C6 whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.

Quick Database Links

Gene Symbol

KLF9

UniProt

Q13886

RefSeq (Protein):NP_001197.1