KLF6 Antibody (C-term)

SKU: orb1929959

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42234
• Immunogen: This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6. Antigen Region: 159-186 aa.
• Target: KLF6
• Molecular Weight: 31865 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Krueppel-like factor 6, B-cell-derived protein 1, Core promoter element-binding protein, GC-rich sites-binding factor GBF, Proto-oncogene BCD1, Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, KLF6, BCD1, COPEB, CPBP, ST12

Flow cytometric analysis of widr cells using KLF6 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of KLF6 Antibody (C-term) in mouse stomach tissue lysates (35 ug/lane). KLF6 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

KLF6

UniProt

Q99612

RefSeq (Protein):NP_001153596.1, NP_001291.3, NP_001153597.1