KLF4 Antibody (C-term)

SKU: orb1937837

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD36578
• Immunogen: This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-421 amino acids from the C-terminal region of human KLF4. Antigen Region: 394-421 aa.
• Target: KLF4 (HGNC:6348)
• Molecular Weight: 54671 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF

Fluorescent confocal image of SY5Y cells stained with KLF4 (C-term) Antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with KLF4 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

KLF4 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

KLF4 Antibody (C-term) western blot analysis in A431, MCF-7 cell line lysates (35 ug/lane). This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).

Quick Database Links

Gene Symbol

KLF4 (HGNC:6348)

UniProt

O43474

RefSeq (Protein):NP_004226.3