KLF15 Antibody

• Catalog Number: orb1247337
• Category: Antibodies
• Description: KLF15 Antibody
• Target: KLF15
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: VDHLLPVDENFSS-C
• UniProt ID: Q9UIH9
• Tested applications: ELISA, FC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44kDa according to NP_054798.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat, Mouse and Pig Kidney, and NIH3T3 for this particular batch.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KLF15, KKLF, Kruppel-like factor 15, KKLF protein,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_054798.1

orb1247337 (1 ug/ml) staining of nuclear HepG2 (A) and (0.5 ug/ml) HeLa (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247337 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).