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Catalog Number | orb1929596 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the C-terminal region of human KIST (KIS). |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Predicted Reactivity | Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | Q8TAS1 |
MW | 46546 Da |
Tested applications | WB |
Dilution range | WB: 1:1000, WB: 1:1000 |
Antibody Type | Primary Antibody |
Clone Number | RB5431 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Serine/threonine-protein kinase Kist, Kinase inter Read more... |
Note | For research use only |
NCBI | NP_001171692.1, NP_653225.2, NP_787062.1 |
The anti-KIST Pab is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
All lanes: Anti-KIST Antibody (L280) at 1:1000 dilution. Lane 1: human skeletal muscle lysate. Lane 2: A-673 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.