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Item 1 of 4
KISS1 Antibody (N-Term)
Catalog Number: orb1926310
| Catalog Number | orb1926310 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB53797 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:500, IHC-P: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14705 Da |
| Target | This KISS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 20-52 amino acids from human KISS1. |
| UniProt ID | Q15726 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Metastasis-suppressor KiSS-1, Kisspeptin-1, Metast Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-KISS1 Antibody (N-Term) at 1:500 dilution + SW480 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-KISS1 Antibody (N-Term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-KISS1 Antibody (N-Term) at 1:2000 dilution. Lane 1: human placenta lysates. Lane 2: human skeletal muscle lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining KISS1 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
