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Item 1 of 3
KISS1 Antibody (C-Term)
Catalog Number: orb1926311
| Catalog Number | orb1926311 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB53799 |
| Tested applications | IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:25, WB: 1:2000, WB: 1:2000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14705 Da |
| Target | This KISS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the human region of human KISS1. |
| UniProt ID | Q15726 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Metastasis-suppressor KiSS-1, Kisspeptin-1, Metast Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-KISS1 Antibody (C-Term) at 1:2000 dilution + HL-60 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-KISS1 Antibody (C-Term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysates. Lane 2: SW480 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KISS1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
