Kir2.1 Antibody (HRP)

SKU: orb148333

Description

Mouse monoclonal to Kir2 (HRP). 1. Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. The Kir2. 1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome....

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1
• Clone No.: S112
• Immunogen: Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
• Target: Kir2.1
• Molecular Weight: 45kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Kir2.1, KCNJ2, Inward rectifier potassium channel 2, Inward rectifier K(+) channel Kir2.1, Potassium channel inwardly rectifying subfamily J member 2, IRK1, HHBIRK1, HHIRK1, HIRK 1, LQT7, SQT3

Quick Database Links

Gene Symbol

Kir2.1

UniProt

P35561

NCBI Gene ID

16518

RefSeq (Protein):NP_032451