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kininogen 1 Antibody

SKU: orb1248301

Description

kininogen 1 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80+60+30kDa bands observed in Human Kidney lysates (calculated MW of 72.0kDa according to NP_001095886.1). The observed MW bands are explained by glycosylation and the splitting into heavy and light chains from the precursor protein. Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-RITEATKTVGSDT
Targetkininogen 1
Molecular WeightApprox 80 + 60 + 30kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KNG1, kininogen 1, BDK, KNG, HMWK, alpha-2-thiol proteinase inhibitor, bradykinin, fitzgerald factor, high molecular weight kininogen, kininogen-1, williams-Fitzgerald-Flaujeac factor

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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