KIFC1 Antibody

SKU: orb1257893

Description

KIFC1 Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2).
• Target: KIFC1
• Molecular Weight: Observed: 74kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

KIFC1, HSET, KNSL2, Kinesin-like 2, Kinesin-related protein, Kinesin family member C1, Kinesin-like protein KIFC1, Kinesin-like protein 2, Kinesin-related protein HSET

Western blot analysis of extracts of various cell lines, using KIFC1 antibody (orb1257893) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using KIFC1 antibody (orb1257893) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using KIFC1 antibody (orb1257893) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using KIFC1 antibody (orb1257893) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2 OS cells using KIFC1 Polyclonal Antibody (orb1257893) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

KIFC1

UniProt

Q9BW19