KIFAP3 Antibody

• Catalog Number: orb1257679
• Category: Antibodies
• Description: KIFAP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 110kDa
• Target: KIFAP3
• UniProt ID: Q92845
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using KIFAP3 antibody (orb1257679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug KIFAP3 antibody (orb1257679). Western blot was performed from the immunoprecipitate using KIFAP3 antibody (orb1257679) at a dilution of 1:1000.