Ki67 Antibody

• Catalog Number: orb1261257
• Category: Antibodies
• Description: Ki67 Antibody
• Target: MKI67
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Ki67 (NP_001139438.1).
• UniProt ID: P46013
• MW: Observed: 359kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: monoclonal Ki-67, Proliferation-related Ki-67 anti
• Note: For research use only

Western blot analysis of extracts of various cell lines, using Ki67 antibody (orb1261257) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using Ki67 antibody (orb1261257).