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Catalog Number | orb27421 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Ki67 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MIB-1 |
Tested applications | IHC-Fr, IHC-P |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Human nuclear Ki-67 antigen |
Antibody Type | Primary Antibody |
Dilution range | (RTU) neat |
Conjugation | Unconjugated |
Hazard Information | *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules! |
UniProt ID | P46013 |
RRID | AB_10755396 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required. |
Note | For research use only |
Application notes | Positive Control: Breast or colon carcinoma. Pre-Treatment: Unmasking fluid G (Art. No. DE007) or C (Art. No. DE000) at 90-100°C. Incubation Time: 60 min at RT |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of using Ki67 antibody
Greater than 90% as determined by SDS-PAGE. | |
56.6 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
17.3 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
36.7 kDa | |
E.coli |