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Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IP, Multiplex Assay, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:20,000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:100, WB: 1:50 - 1:200 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | C11 |
| Immunogen | This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells. |
| Target | KRT6C |
| Purity | This protein A purified mouse monoclonal antibody reacts specifically with keratins from human tissues and derived cell lines. This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans. Cross reactivity with keratins from other sources has not been determined. No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.3 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-keratin antibody, mouse anti-cytokeratin 6 antibody, PC2 antibody, Type II keratin isoform K6c antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation




















