keratin 18 Antibody

SKU: orb1248253

Description

keratin 18 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kDa according to NP_000215.1) and approx 45kDa in lysates of Pig Liver (calculated MW of 47.4kDa according to XP_005652636.2). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:Paraffin embedded Human Breast, Placenta and Small Intestine. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EELDKYWSQQIE
• Target: keratin 18
• Molecular Weight: Approx 48kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT18, keratin 18, CYK18, K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, cytokeratin 18, cytokeratin-18, keratin, type I cytoskeletal 18, keratin-18

orb1248253 (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248253 (0.1 ug/ml) staining of Pig Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb1248253 (5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248253 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1248253 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

keratin 18

UniProt

P05783

RefSeq (Protein):NP_000215.1