KEAP1 Antibody

• Catalog Number: orb1249275
• Category: Antibodies
• Description: KEAP1 Antibody
• Target: KEAP1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-EVTPSQHGNRTFS
• UniProt ID: Q14145
• Tested applications: ELISA, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KEAP1, kelch-like ECH-associated protein 1, INrf2,
• Note: For research use only
• NCBI: NP_036421.2

orb1249275 (0.2 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249275 (3.8 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249275 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.