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    Kdm6b Antibody

    Catalog Number: orb1260760

    DispatchUsually dispatched within 5-10 working days
    $ 551.00
    Catalog Numberorb1260760
    CategoryAntibodies
    DescriptionKdm6b Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, WB
    ReactivityHuman, Mouse
    IsotypeIgG
    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1).
    Concentrationbatch dependent
    Dilution rangeWB: 1:500 - 1:2000IF: 1:50 - 1:200
    Form/AppearanceLiquid
    ConjugationUnconjugated
    MWObserved: 170kDa
    TargetKdm6b
    StorageStore at -20°C. Avoid freeze / thaw cycles.
    Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
    Alternative namesLysine-specific demethylase 6B, 11411-, JmjC domai
    Read more...
    NoteFor research use only
    Application notesWB: 1:500 - 1:2000IF: 1:50 - 1:200
    Expiration Date12 months from date of receipt.
    Kdm6b Antibody

    Western blot analysis of extracts of various cell lines, using Kdm6b antibody (orb1260760) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

    Kdm6b Antibody

    Western blot analysis of extracts of mouse brain, using KDM6B antibody (orb1260760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

    Kdm6b Antibody

    Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody (orb1260760) at dilution of 1:100. Blue: DAPI for nuclear staining.

    Kdm6b Antibody

    Immunofluorescence analysis of U2OS cells using KDM6B antibody (orb1260760) at dilution of 1:100. Blue: DAPI for nuclear staining.

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