KDM4B Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1172985
• Category: Antibodies
• Description: KDM4B Recombinant Rabbit Monoclonal Antibody
• Target: KDM4B
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• MW: 122 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: G8C10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KDM4B_HUMAN; Lysine-specific demethylase 4B; JHDM3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: T47D, 2: LNCap, 3: K562, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 122 kDa, Observed MW: 150 kDa.

Cell line: K562, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172985.

Cell line: K562, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172985.