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Catalog Number | orb1256070 |
---|---|
Category | Antibodies |
Description | KDM4A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 150kDa |
Target | KDM4A |
UniProt ID | O75164 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, J Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using KDM4A antibody (orb1256070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using KDM4A antibody (orb1256070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (orb1256070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (orb1256070) at dilution of 1:100 (40x lens).
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Human, Rat, Sheep | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |