KDEL Receptor Antibody

SKU: orb1822492

Description

Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1

Research Area

Cell Biology, Disease Biomarkers, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: KR-10
• Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
• Target: KDEL Receptor
• Molecular Weight: 25kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KDELR1, KDEL receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, ER lumen protein-retaining receptor 1, ERD2, ERD2.1, ERD21, KDEL R1, PM23, HDEL

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images.

Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

KDEL Receptor

UniProt

P33946

NCBI Gene ID

618184

RefSeq (Protein):NP_001069963.1