KDEL Rabbit Polyclonal Antibody (BF647)

SKU: orb1600941

Description

KDEL Rabbit Polyclonal Antibody (BF647) is a BF647 conjugated antibody targeting POGLUT2. This antibody is suitable for IF. It exhibits reactivity with Mouse, Rat samples.

Images & Validation

• Tested Applications: IF
• Dilution Range: IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Guinea pig, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide KDEL
• Target: POGLUT2
• Purification: Affinity purified by Protein A
• Conjugation: BF647

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KDEL; Lys Asp Glu Leu antibody; Lys-Asp-Glu-Leu.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-KDEL antibody (orb1600941), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-KDEL/AF647 Conjugated antibody (orb1600941), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

POGLUT2