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KDEL Antibody: PerCP

Catalog Number: orb151347

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200 μg$ 700.00
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Catalog Numberorb151347
CategoryAntibodies
DescriptionRabbit polyclonal to KDEL (PerCP). The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER..
TargetKDEL
ClonalityPolyclonal
Species/HostRabbit
ConjugationPerCP
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/Preservatives95.64mM Phosphate, 2.48mM MES and 2mM EDTA
PurificationProtein A Purified
ImmunogenKDEL containing peptide immunogen
Tested applicationsELISA, ICC, IF, IHC, WB
Dilution rangeWB (1:1000), ICC/IF (1:100)
Application notesA 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
StorageConjugated antibodies should be stored according to the product label
Alternative namesLys Asp Glu Leu antibody
Research AreaCell Biology, Disease Biomarkers, Protein Biochemi
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NoteFor research use only
Expiration Date12 months from date of receipt.
KDEL Antibody: PerCP

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

KDEL Antibody: PerCP

Western blot analysis of Human Cell line lysates showing detection of KDEL protein using Rabbit Anti-KDEL Polyclonal Antibody. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:1000, 1:500.

KDEL Antibody: PerCP

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

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    ELISA,  ICC,  IF,  IHC,  WB

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