KDEL Antibody

SKU: orb1822445

Description

Rabbit Anti-KDEL Polyclonal

Research Area

Cell Biology, Disease Biomarkers, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KDEL containing peptide immunogen
• Target: KDEL
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KDEL, K-D-E-L, Lys-Asp-Glu-Leu, Lys-Asp-Glu-Leu (KDEL), Lysine-aspartic acid-glutamate-leucine

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of Human Cell line lysates showing detection of KDEL protein using Rabbit Anti-KDEL Polyclonal Antibody. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:1000, 1:500.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Quick Database Links

Gene Symbol

KDEL