[KD Validated] eIF4E Rabbit pAb Discontinued

SKU: orb48359

Description

Rabbit polyclonal antibody to EIF4E

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, IP, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-100ICC1:50-200, IP: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).
• Molecular Weight: 25kDa/28kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EIF4E1 antibody, anti EIF4EL1 antibody, anti EIF4F antibody, anti MGC111573 antibody

Immunofluorescence analysis of U2OS cells using [KD Validated] eIF4E Rabbit pAb (orb48359) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KD Validated] eIF4E Rabbit pAb (orb48359) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human stomach using [KD Validated] eIF4E Rabbit pAb (orb48359) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200 µg extracts of 293T cells, using 3 µg [KD Validated] eIF4E Rabbit pAb (orb48359). Western blot was performed from the immunoprecipitate using [KD Validated] eIF4E Rabbit pAb at a dilution of 1:1000.

Western blot analysis of lysates from wild type (WT) and eIF4E knockdown (KD) 293T cells using [KD Validated] eIF4E Rabbit pAb (orb48359) at 1: 1400 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 10 s.

Western blot analysis of various lysates, using [KD Validated] eIF4E Rabbit pAb (orb48359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Quick Database Links

UniProt

P06730

Yang, Xiaodan et al. Picornavirus 2A protease regulates stress granule formation to facilitate viral translation PLoS Pathog, 14, e1006901 (2018)

PubMed: 29415027