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Validated
Description
Research Area
Images & Validation
−| Tested Applications | IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | BT27X0K5 |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser657 of human PKC alpha |
| Target | PRKCA |
| Molecular Weight | Calculated MW: 77 kDa; Observed MW: 80 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Concentration | 0.3 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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![[KD] Phospho-PKC alpha (Ser657) Rabbit mAb](/images/pub/media/catalog/product/NewWebsite/21/orb1563989_1.jpg)
Western blot analysis of Phospho-PKC alpha (Ser657) in C6, rat brain lysates using Phospho-PKC alpha (Ser657) antibody.
![[KD] Phospho-PKC alpha (Ser657) Rabbit mAb](/images/pub/media/catalog/product/NewWebsite/21/orb1563989_2.jpg)
Western blot analysis of PKC alpha (Phospho-Ser657) in mouse Brain, Ramos lysates using PKC alpha (Phospho-Ser657) antibody.
![[KD] Phospho-PKC alpha (Ser657) Rabbit mAb](/images/pub/media/catalog/product/NewWebsite/21/orb1563989_3.jpg)
Immunohistochemistry analysis of paraffin-embedded Human brain using Phospho-PKC alpha (Ser657) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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UniProt Details
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Protocol Information
[KD] Phospho-PKC alpha (Ser657) Rabbit mAb (orb1563989)
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