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Catalog Number | orb1936764 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB33321 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 88984 Da |
Target | This KCTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 725-754 amino acids from the C-terminal region of human KCTD3. |
UniProt ID | Q9Y597 |
NCBI | NP_057205.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | BTB/POZ domain-containing protein KCTD3, Renal car Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-KCTD3 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
KCTD3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of KCTD3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
KCTD3 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the KCTD3 antibody detected the KCTD3 protein (arrow).