KCNQ1 Antibody (HRP)

SKU: orb148282

Description

Mouse monoclonal to KCNQ1 (HRP). Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifically, Kv7. 1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. Kv7. 1 is present in the cell membranes of cardiac muscle tissue and in inner ear neurons among other tissues. In the cardiac cells, Kv7. 1 mediates the IKs (or slow delayed rectifying K+) current that contributes to the repolarization of the cell, terminating the cardiac action potential and thereby the heart's contraction...

Research Area

Cell Biology

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Hamster, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of KCNQ1 in 10 µg of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N37A/10 (Formerly sold as S37A-10)
• Immunogen: Fusion protein amino acids 2-101 of human KCNQ1
• Target: KCNQ1
• Molecular Weight: 75kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KCNQ1, Kv7.1, KVLQT1, Potassium voltage-gated channel subfamily KQT member 1, Voltage-gated potassium channel subunit Kv7.1, KQT-like 1, IKs producing slow voltage-gated potassium channel subunit alpha, Slow delayed rectifier channel subunit, ATFB1, ATFB3, FLJ26167, JLNS1, KCNA8, KCNA9, KCNQ1_HUMAN, Kidney and cardiac voltage dependent K+ channel, Kv1.9, Long (electrocardiographic) QT syndrome, LQT, LQT1, RWS, SQT2, Ward-Romano syndrome 1

Quick Database Links

Gene Symbol

KCNQ1

UniProt

P51787

NCBI Gene ID

3784

RefSeq (Protein):NP_000209.2