KCC2 Antibody

SKU: orb67480

Description

Mouse Anti-Rat KCC2 Monoclonal IgG2a

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC-P (1:300), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N1/12 (Formerly sold as S1-12)
• Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2
• Target: KCC2
• Molecular Weight: 140kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KCC2, KCC 2, rKCC2, hKCC2, SLC12A5, S12A5, KIAA1176, Solute carrier family 12 member 5, Solute carrier family 12 (potassium chloride transporter) member 5, Potassium chloride transporter 5, Electroneutral potassium-chloride cotransporter 2, Electroneutral potassium chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, Furosemide sensitive K Cl cotransporter, K-Cl cotransporter 2, Neuronal K-Cl cotransporter, Neuronal K Cl cotransporter, Erythroid K Cl cotransporter 2, Potassium Chloride Cotransporter, GRP78_HUMAN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2 Monoclonal Antibody, Clone N1/12. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2 Monoclonal Antibody, Clone N1/12. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) KCC2 Antibody. (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone N1/12. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

KCC2

UniProt

Q63633

NCBI Gene ID

171373

RefSeq (Protein):NP_599190