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Catalog Number | orb1258213 |
---|---|
Category | Antibodies |
Description | KAT2A Antibody |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KAT2A (NP_066564.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 110kDa |
Target | KAT2A |
UniProt ID | Q92830 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Histone acetyltransferase KAT2A, General control o Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using KAT2A antibody (orb1258213) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat liver using KAT2A antibody (orb1258213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using KAT2A antibody (orb1258213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using KAT2A antibody (orb1258213) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using KAT2A Polyclonal Antibody (orb1258213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using KAT2A Polyclonal Antibody (orb1258213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KAT2A Polyclonal Antibody (orb1258213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |