Kartogenin

SKU: orb1307547

Description

Kartogenin is a small molecule that activates the Smad4/5 pathway to direct mesenchymal stem cell chondrogenesis. It is utilized in both in vitro and in vivo cartilage regeneration research, offering a tool for studying osteoarthritis therapies and tissue engineering.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 4727-31-5
• MW: 317.34
• Purity: 97.79% (May vary between batches)
• Formula: C₂₀H₁₅NO₃
• SMILES: OC(=O)c1ccccc1C(=O)Nc1ccc(cc1)-c1ccccc1
• Target: TGF-beta/Smad
• Solubility: H2O:<1 mg/mL;10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.3 mM);DMSO:63 mg/mL (198.53 mM);Ethanol:18 mg/mL (56.72 mM)

Bioactivity

• In Vivo:
  Kartogenin at a concentration of 100 nM stimulates collagen synthesis in the dermis of mice. Additionally, it promotes the growth of skeletal limbs and the development of synovial joints in mice. Concentrations of 10 μM and 100 μM Kartogenin respectively enhance cartilage repair in mouse models of osteoarthritis (OA) induced by collagenase VII and surgically.
• In Vitro:
  Kartogenin promotes type I collagen synthesis in dermal fibroblasts by activating the Smad4/Smad5 pathway, without exhibiting significant cytotoxicity on the activity, morphology, and survival of fibroblasts. Additionally, by enhancing the nuclear localization of CBFβ, Kartogenin induces differentiation of hMSCs into chondrocytes and protects articular chondrocytes.
• Cell Research:
  For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer's instructions, cell viability and viability are assessed by AlamarBlue assay.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cells, chondrocytes, differentiation, mesenchymal, KGN, inhibit, Kartogenin, Inhibitor, osteoarthritis, human, TGF-beta, TGFbeta/Smad, TGF-beta/Smad, TGFbeta, TGF-β/Smad, TGFβ, TGF-b/Smad, TGFb, Transforming growth factor beta, Smad, Smad5, Smad4, stem

Compound Identifiers

PubChem CID

2826191